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pet30a 1 expression vector  (New England Biolabs)


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    New England Biolabs pet30a 1 expression vector
    Pet30a 1 Expression Vector, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 4940 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pet30a+1+expression+vector/NdeI/10__1128_slash_mSphere__00934___21-164-23-32
    Average 99 stars, based on 4940 article reviews
    pet30a 1 expression vector - by Bioz Stars, 2026-10
    99/100 stars

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    Related Articles

    Amplification:

    Article Title: Optimization of Multivalent Gold Nanoparticle Vaccines Eliciting Humoral and Cellular Immunity in an <i>In Vivo</i> Model of Enterohemorrhagic Escherichia coli O157:H7 Colonization
    Article Snippet: EHEC (EDL933) genomic DNA was isolated via a Qiagen DNeasy blood and tissue kit according to the manufacturer’s directions. .. Sequences encoding LomW (GI:12514345), EscC (GI:12518466), LpfA1 (GI:12518278), and LpfA2 (GI:12518581) were amplified via Phusion polymerase (New England BioLabs) and cloned into a pET30a(1) expression vector using NdeI and XhoI or HindIII-HF (New England BioLabs) restriction sites. ..

    Clone Assay:

    Article Title: Optimization of Multivalent Gold Nanoparticle Vaccines Eliciting Humoral and Cellular Immunity in an <i>In Vivo</i> Model of Enterohemorrhagic Escherichia coli O157:H7 Colonization
    Article Snippet: EHEC (EDL933) genomic DNA was isolated via a Qiagen DNeasy blood and tissue kit according to the manufacturer’s directions. .. Sequences encoding LomW (GI:12514345), EscC (GI:12518466), LpfA1 (GI:12518278), and LpfA2 (GI:12518581) were amplified via Phusion polymerase (New England BioLabs) and cloned into a pET30a(1) expression vector using NdeI and XhoI or HindIII-HF (New England BioLabs) restriction sites. ..

    Expressing:

    Article Title: Optimization of Multivalent Gold Nanoparticle Vaccines Eliciting Humoral and Cellular Immunity in an <i>In Vivo</i> Model of Enterohemorrhagic Escherichia coli O157:H7 Colonization
    Article Snippet: EHEC (EDL933) genomic DNA was isolated via a Qiagen DNeasy blood and tissue kit according to the manufacturer’s directions. .. Sequences encoding LomW (GI:12514345), EscC (GI:12518466), LpfA1 (GI:12518278), and LpfA2 (GI:12518581) were amplified via Phusion polymerase (New England BioLabs) and cloned into a pET30a(1) expression vector using NdeI and XhoI or HindIII-HF (New England BioLabs) restriction sites. ..



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    Bacterial strains and plasmids used in this study.
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    Bacterial strains and plasmids used in this study.

    Journal: Canadian Journal of Veterinary Research

    Article Title: Protective efficacy of a Salmonella Typhimurium ghost vaccine candidate constructed with a recombinant lysozyme–PMAP36 fusion protein in a murine model

    doi:

    Figure Lengend Snippet: Bacterial strains and plasmids used in this study.

    Article Snippet: Escherichia coli isolates HJL505 and BL21(DE3) (Invitrogen, Carlsbad, California, USA) with the pET30a plasmid (an expression vector inducible by isopropyl β-D-1-thiogalactopyranoside) (Novagen, Temecula, California, USA) containing the genes for the recombinant fusion protein were used in overexpression of the fusion protein ( ).

    Techniques: Plasmid Preparation, Expressing